Dhanalakshmi Marimuthuand Angayarkanni Jayaraman2*

 1Department of Botany, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore- 641043, India.
2Department of Microbial biotechnology, School of Biotechnology, Bharathiar University, Coimbatore 641046, India.

Abstract

In the present study the freshwater algae Chlorosarcinopsis eremi was isolated from the water sample of Kovai Kutralum. The pure culture of the algae was obtained using serial dilution technique followed by streak plate method. The species was observed under light microscopy and identified with the help of algal manuals and also by sequencing 18S rRNA genes. The growth of the algae, C. eremi was characterized in different growth media. The different growth media’s include Bold’s Basal medium, Modified Bourrelly medium, BG 11 medium, Half strength Chu10 medium and Modified Hoagland’s medium. The growth was measured by observed optical density of the biomass and chlorophyll “a” content. The experiment was carried out three times and mean values and standard deviation were calculated. From the results it is concluded that growth of C. eremi was maximum in Bold’s basal medium when compared with other media tested.

Keywords: Chlorosarcinopsis eremi; 18SrRNA; Growth characteristic; Bold’s basal medium.