IrdaSayuti1, Yusni Ikhwan Siregar1, Bintal Amin1, Anthoni Agustien2 and Akmal Djamaan3*

 1Department of Enviromental Science, University of Riau, Riau. Indonesia.
2Department of Biology, Faculty of Mathematic and Natural Science, University of Andalas, Padang-West Sumatra, Indonesia.
3Laboratory of BiotechnologyBiotan Sumatra/Faculty of Pharmacy, University of Andalas, Padang, West Sumatra, Indonesia.


This research was conducted to identify the bacterial hydrocarbonclastic in waste tanks, Petapahan, Riau, Indonesia. Bacteria hydrocarbonoclastic are bacteria that have traffic in degrade petroleum. Analysis of 16S rRNA used the primers pair 63 Forward and Reverse 1387 produced 1300 bp. Results of the analysis of the 16Sr RNA sequence in bacterial isolates obtained that IMB-0909 was similar to Pseudomonas tollasii, IMB-10 was similar to Bacillus cereus IMB11 had similarities with Bacillus toyonensis strain BCT-7112, IMB 12 was Lysinobacillus fusiform strainNBRC15717, IMB 15 was Pseudomonas stutzeri strain ATCC 17588 entirely did have the ability to degradepetroleum.

Keywords: 16S RNA, hydrocarbonoclastic, bacteria, waste tank, petroleum.