Identification of Bacterial Hydrocarbonoclastic in Waste Tanks, Petapahan, Riau, Indonesia, using 16sr RNA

ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access

Irda Sayuti¹, Yusni Ikhwan Siregar¹, Bintal Amin¹, Anthoni Agustien² and Akmal Djamaan³

¹Department of Enviromental Science, University of Riau, Riau, Indonesia.

²Department of Biology, Faculty Mathematic and Natural Science, Andalas University, Padang-West Sumatra, Indonesia.

³Laboratory of Biotechnology Biotan Sumatra/Faculty of Pharmacy, Andalas University, Padang-West Sumatra, Indonesia.

J Pure Appl Microbiol. 2018;12(2):671-677

https://doi.org/10.22207/JPAM.12.2.25 | © The Author(s). 2018

Received: 04/03/2018 | Accepted: 20/04/2018 | Published: 30/06/2018

Abstract

This research was conducted to identify the bacterial hydrocarbonclastic in waste tanks, Petapahan, Riau, Indonesia. Bacteria hydrocarbonoclastic are bacteria that have traffic in degrade petroleum. Analysis of 16S rRNA used the primers pair 63 Forward and Reverse 1387 produced 1300 bp. Results of the analysis of the 16S rRNA sequence in bacterial isolates obtained that IMB- 09 09 was similar to Pseudomonas tollasii, IMB- 10 was similar to Bacillus cereusIMB 11 had similarities with Bacillus toyonensis strain BCT-7112, IMB 12 was Lysinobacillus fusiform strain NBRC 15717, IMB 15 was Pseudomonas stutzeri strain ATCC 17588 entirely did have the ability to degrade petroleum.

Keywords

16S rRNA, Hydrocarbonoclastic, Bacteria, Waste tank, Petroleum

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