Sarab Hussein Khaleel1, Ibtisam H. Al-Azawia2 and
Ali Hussein Khlebos3

1Department of Anatomy, College of Medicine. University of Al- Qadisiyah, Diwaniyah, Iraq.
2Department of Microbiology, College of Medicine. University of Al- Qadisiyah, Diwaniyah, Iraq.
3Iraq Ministry Healthy, Al-Dewany Teaching Hospital, Laboratory Department, Diwaniyah, Iraq.


Cholera is one of the most important epidemic diseases globally. It is causing of morbidity and mortality in the world. Severe watery diarrhea causes a composite process involving several component that help them reach the lining of the small intestine, form colonies and generate bacterial toxins.This survey was carried out to discover the genetic patterns of clinical isolates according to the presence or absence of toxic genes for the city of Diwaniyah in southern Iraq. Sixty isolates were isolated from patients with cholera. The isolates included the center of the city and the surrounding rural areas. Biochemical and serological diagnosis. All the isolates were V. cholera  serogroup O 1 of the serotyping  Ogawa in  biotype El Tor  . Genetic testing was carried out using PCR technique and base on the presence or absence of toxin genes. Three genotypes were identified for the region.

Keywords: Vibrio, Cholera, biochemical and surgical diagnosis.