ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access

Sagar Mali, P.M. Beena and N. Arvind

Department of Microbiology, Sri Devaraj Urs Medical College, Tamaka, Kolar, Karnataka, India.
J Pure Appl Microbiol. 2018;12(2):871-877
https://doi.org/10.22207/JPAM.12.2.49 | © The Author(s). 2018
Received: 11/04/2018 | Accepted: 09/05/2018 | Published: 30/06/2018
Abstract

Extra pulmonary tuberculosis presents a diagnostic dilemma for both physicians as well as for clinical microbiologists . The laboratory diagnosis of tuberculosis ranges from simple microscopy,culture to complex molecular assays. To evaluate the sensitivity, specificity and turnaround time of microscopy, culture and PCR in the diagnosis of Extrapulmonary tuberculosis & to evaluate the use of PCR in the early diagnosis of Extrapulmonary tuberculosis. A total of 71  samples patients with strong clinical suspicion of extra-pulmonary tuberculosis were processed and evaluated by ZN staining, fluorescent microscopy, LJ culture, BacT Alert  culture and PCR. The positivity rates by microscopy, LJ culture, BacT Alert  culture and PCR were 11.26%, 8.45%, 14.08% and 14.08% respectively. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) of both staining methods was 50%, 92.3%, 37.5% and 95.2% respectively. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) of BacT Alert  culture was 83.3%, 92.3%, 50% and 98.4% respectively. The recovery rate was higher by BacT Alert  culture (90.9%) compared to LJ culture (63.63%). The mean turnaround time for culture positivity was 36.3 days with LJ culture and 14.6 days with BacT Alert  culture. The sensitivity, specificity, positive predictive value and negative predictive value of PCR assay was 66.66%, 90.76%, 40% and 96.72% respectively. PCR has high sensitivity, specificity, substantial level of agreement with BacT Alert culture and shorter turnaround time. Therefore, use of PCR in combination with other diagnostic modalities is a useful tool to detect additional EPTB cases which may be missed otherwise.

Keywords

Extrapulmonary tuberculosis, diagnostic methods.

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