Isolation and Identification of Pathogenic Bacteria from Vaginal Cavity of Sheep and Goats in Iraq

This study intends to explore the prevalence of pathogenic bacteria infected the vaginal cavity of sheep and goats, in addition to determine the normal vaginal bacterial flora in pregnant and non pregnant local breed goat and sheep. Vaginal swabs were collected from 80 cases comprised of 54 sheep and 26 goats that involve 20 and 6 pregnant ewes and goats from Baghdad city during May 2019 to July 2019. These samples were cultivated onto different bacteriological media. The isolated bacteria were identified by different biochemical tests. Mixed bacterial isolates were determined in 65 out of 80 (81.3%) samples. Totally, 9 various microorganisms were isolated from animal’s vagina. E. coli, Streptococcus Spp, KlebsiellaSpp and Staphylococcus aureus were the most common isolated microorganism from vaginal swabs in a percentage of 36, 20, 17.5 and 16.3%respectively. While Bacillus Spp, Proteus Spp, Staphylococcus Spp, Corynebacterium Spp and Salmonella Spp.were the less common isolated microorganisms in a percentages of 10, 10, 7.5, 6.3 and %2.5 respectively. Moreover, the enteric bacteria especially E. coli was isolated in the higher incidence rate (44.4%) in pregnant ewes compared to pregnant goats 15.4%. However, in non-pregnant animals, the percentages were 50 and 15% in sheep and goats respectively. Nonetheless, Proteus Spp were isolated only from newly birth animals. Meanwhile, Staphylococcus aureus were relatively isolated at the same level of incidence at 15%-17% in either pregnant or non-pregnant sheep or goats, while Streptococcus Spp were mostly found in pregnant goats and ewes at 50 and 25% respectively. In conclusion, this study approved the isolation of different bacterial spp in various incidence rates from vaginal cavity of pregnant and nonpregnant sheep and goats.


INTRODUCTION
The importance of reproductive efficiency in the sheep is very essential because of the seasonal breeding. Many diseases are classified as specific and non-specific which cause problems in the reproductive system in the sheep. Some of these diseases cause infertility or reduce productivity with clinical signs which may include vaginal discharge. Abortion and infertility can result from infection or from non-infectious causes (El-Arabi et al., 2013).
Genital infections in domestic ruminants are often caused by opportunistic secondary invaders, especially Escherichia coli spp that have frequently been isolated in majority from ewes (Sargison et al., 2007 andManes et al., 2010), goats (Ababneh and Degefa, 2006). Coliforms (of fecal origin) and other non-specific bacteria are also opportunistic pathogens in the reproductive tract. Under stressful conditions, these opportunist bacteria may cause genital infection that usually leads to reproductive failure in ruminants (Shallali et al., 2001 andMshelia et al., 2014).Vaginitis, a common disease of the genital tract of domestic ruminants, is often caused by secondary bacterial invaders, mainly the E. coli species (Sargison et al., 2007 andManes et al., 2010).
These microorganisms caused diseases due to stress and reduction of the immunity of

MATERIAL AND METHODS Collection of samples
For bacterial isolation, 80 vaginal swabs were collected aseptically from 54 sheep and 26 goats from different areas in Baghdad city. All samples were collected using disposable sterile swabs with transport media (Greiner). The out surface of vagina was disinfected by 70% alcohol then the swabs were introduced in vaginal cavities to collect the samples. These swab samples were cultured on different cultural media and incubated at 37°C for 24-48 hrs. All bacterial isolates were identified by cultural characteristic features of colonies on primary and specific media in addition to gram staining and according to the results of biochemical tests. (Quinn et al., 2004).

Cultural media
Different cultural media were used to cultivate the samples including blood agar, MacConky agar, Mannitol salt agar, Eosin Methylene Blue agar, nutrient agar, tetrathionate broth and SS agar and incubated at 37°C for 24-48 hrs.

Biochemical tests
Many different biochemical tests were done to identify the bacterial isolates including catalase test, coagulase test, IMVIC test, TSI test and motility test.

RESULT AND DISCUSSION
In this study, the results were revealed that nine bacterial isolates were identified in various percentages in sheep and goats.  The results of the current study revealed the isolation of different percentages of microorganisms in pregnant and non-pregnant ewes and does table (2 & 3). These results are compatible with other researcher (Martins et al., 2009), who stated that the alteration on microflora was due to changes of vaginal environment such as pH modifications during estrus and pregnancy.
The presence of these microorganisms in the vagina as we isolated in this study may cause bacterial vaginitis following the exposure to any factors causing depression of the immunity system In conclusion, this study approved the isolation of different bacterial spp in various incidence rates from vaginal cavity of pregnant and non-pregnant sheep and goats. Totally, 9 various microorganisms were isolated from animals vagina. According to our conclusion we recommended to follow hygienic precautions in intra vaginal implantation to minimize the contamination and avoid the infection with secondary bacterial invaders in addition to reduce the chance of infertility.