Journal of Pure and Applied MicrobiologyVol. 6 No. 4

Antimycotic Potential of Bacterial Ethanolic Extract Isolated from Acid Soil of Mahishapat, Odisha

S.K. Nayak1, G.H. Santra2 and B.B. Mishra1*

1Department of Microbiology, Orissa University of Agriculture and Technology, Bhubaneswar- 751 003, India. 2Department of Soil Science and Agricultural Chemistry, College of Agriculture, OUAT, Bhubaneswar- 751 003, India.

Received on 06 October 2012 and accepted on 19 November 2012

 

ABSTRACT

In view of the large tract of acid soil in India, an attempt was made in the present investigation to study the antimicrobial potential of bacterial flora from acid soil of Mahishapat, Dhenkanal, Odisha. Four bacteria isolated from the soil sample (pH 5.3 ± 0.10) showed wide range of pH tolerance (pH 5.0 to 8.0) were maintained and selected for further study. After biochemical characterizations of the four isolates were identified as Gram positive rods viz., Bacillus subtilis, Bacillus brevis, Bacillus azotofermans and Bacillus licheniformis. Two of the isolates, Bacillus subtilis and Bacillus azotofermans were found to have antimycotic activities against dermatophytes like on Candida albicans MTCC No.854 and Cryptococcus neoformans MTCC No. 1347 respectively. When the organism showed an inhibition zone of 22 mm and 17 mm while the ethanol extract showed 26mm and 21 mm against the test pathogens respectively. It was larger than the medicines recommended by physicians.

Keywords : Acid soil, Bacteria, Ethanol extract, Antimycotic activity, Dermatophytes.