Journal of Pure and Applied MicrobiologyVol. 6 No. 4

Evaluation of Phosphate Solubilizing Pseudomonas Strains and their Mutants in Relation to Mustard (Brassica compestris)

Meenu Rathi1, Deepak Kumar Malik2 and Baljit Singh Dahiya3

1Department of Botany, University college, Kurukshetra University, Kurukshetra - 136 119, India. 2University Institute of Engineering and Technology, Kurukshetra University Kurukshetra, India. ?Department of Botany, Janta Vedic College, Baraut - 250 611, India.

Received on 05 May 2012 and accepted on 08 June 2012

 

ABSTRACT

Phosphorous is involved in many essential biochemical processes like cell division, photosynthesis, sugar break down, transfer of energy and nutrient uptake. Two phosphate solubilizing pseudomonas strains 25MRP and 33MRP were taken in this study. Tn-5 derived mutants of 25MRP and 33MRP were obtained with altered phosphate solubilizing ability by using E.coli strain S17-1. Highest phosphate solubilization and maximum drop in pH were found with 33MRP-314 mutant. Under pot house conditions maximum plant dry biomass was observed with 33MRP-233 with URP (3.43g/pl) followed by 25MRP-61 with URP (3.32 g/pl) at 60 DAS. The phosphate uptake ranged between (11-298%), and maximum phosphorous uptake observed in 25MRP-233 with URP (0.726 mg/pl) followed by 25MRP-61 with URP (0.712 mg/pl). Better performance with low phosphate solubilizing mutants such as 25 MRP-179 and 33MRP-188 in relation to plant dry weight indicated that some other metabolites may also be involved in plant growth promotion.

Keywords : Phosphate solubilization, Seed bacterization, Mustard, Mutation.