Journal of Pure and Applied MicrobiologyVol. 6 No. 4

Protein Profile of Clostridium perfringens Isolated from Animals and Foods of Animal Origin

S. Mehtaz*, P. Borah, and R.K. Sharma

Department of Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati- 781 022, India.

Received on 02 April 2012 and accepted on 10 May 2012

 

ABSTRACT

Partially purified protein extracts from six randomly selected isolate was subjected to SDS-PAGE to study their protein profile. The isolates exhibited variability in protein profiles in terms of both number of bands and corresponding molecular weights. Five of the preparations were found to share a common protein band with almost similar molecular weights within the range of 33.4 kDa and 34.8 kDa, which indicated production of alpha toxin by the concerned isolates. All these isolates also showed production of DNase and phospholipase C, while haemolysin production was observed in three. In addition three isolates also exhibited a common protein band within the range of 43.1 kDa to 44.6 kDa.

Keywords : Clostridium perfringens, DNase, haemolysin, phospholipase C, SDS-PAGE.