Journal of Pure and Applied MicrobiologyVol. 6 No. 4

PCR as a Faster Mode of Supportive Tool in Laboratory Diagnosis of Mycobacterium tuberculosis

Saroj Golia, Vivek Hittinahalli, Mallika Reddy and Sujatha Karjigi

Dr. B.R. Ambedkar Medical College , K.G. Halli, Bangalore - 560 045, India.

Received on 05 July 2012 and accepted on 11 August 2012

 

ABSTRACT

Conventional methods such as microscopy and culture lack the sensitivity and are time consuming.PCR provides good rate of positive results and better turnaround time than culture (days versus weeks) and smear examination. The present study was to shows the sensitivity and time taken for PCR verses positive culture in the diagnosis of tuberculosis .Molecular diagnosis of tuberculosis by PCR has a great potential to improve the clinicians ability to diagnose tuberculosis, so that early treatment to patients can prevent further spread. This study has demonstrated that MTB PCR assay is rapid, sensitive and highly specific as compared to commonly used conventional techniques. In addition, rapidity of the test allows quick implementation of treatment regimen.

Keywords : Mycobacterium tuberculosis, PCR, Nested PCR.