Journal of Pure and Applied MicrobiologyVol. 2 No. 2

Diversity of Microbial Thermophiles in a Neutral Hot Spring (Kawah Hujan A) of Kamojang Geothermal Field, Indonesia

Heni Yohandini1, Fida Madayanti1, Pingkan Aditiawati2 and Akhmaloka1*

ıBiochemistry Research Division, Faculty of Mathematic and Natural Sciences, Institute Teknologi Bandung, Jln Ganesha 10, Bandung. Indonesia. ²School of Life Science and Technology. Institut Teknologi Bandung, Jl Ganesha 10, Bandung, Indonesia.

Received on 18 April 2008 and accepted on 03 July 2008

 

ABSTRACT

Thermophilic microbial community in one of Kamojang hot springs, namely Kawah Hujan A, was analysis through 16S rRNA gene sequences combined with denaturing gradient gel electrophoresis (DGGE). The analysis was based on culture-independent and culture dependent strategies. Kawah Hujan A is a neutral hot spring with pH at 7.3 and low concentration of cation and anion. Microbial communities obtained from culture-independent were close to g-proteobacteria, however, detail comparison among the member of g-proteobacteria showing some sequences variation compared to the published data especially on the hypervariable and variable regions. In addition, the sequences did not belong to certain genus. Meanwhile, the 16S rDNA sequences from culture-dependent samples revealed different sequences compared to that from the culture independent sample. Phylogenetic and homological analysis of the sequences from cultivation samples showed that the sequences were close to Firmicute and Deinococcus phyla, including Geobacillus, Anoxybacillus and Thermus genera.

Keywords : Thermophiles, 16S rRNA, DGGE, community analysis.