Journal of Pure and Applied MicrobiologyVol. 6 No. 4

A Comparative Study on Efficacy of Potential Bio-control Agents in Dynamic Environments

Deepak Rajendran and Jayapradha Ramakrishnan*

School of Chemical and Biotechnology, SASTRA University, Thanjavur - 613 401, India.

Received on 29 April 2012 and accepted on 07 June 2012

 

ABSTRACT

The application of the bio-control agent is on a significant rise in the current agricultural practices. Besides causing no harm to the external environment, these compounds also help in controlling the growth of pathogens. In this study, we have compared the efficiency of Trichoderma viride which is being used in the field over the past few years as a bio-control agent, with the novel Streptomyces species IF5 (GenBank Accession number FJ951435) by analysing the secondary metabolites for anti-microbial activity under various environmental conditions. Several tests were performed to quantify the effect of the metabolites produced from both the strains under varying pH, salt concentration, heavy metals and pesticide endosulfan. We found that the activity of enzymes produced by Streptomyces IF5 was significantly higher than that of Trichoderma viride and also Streptomyces IF5 could tolerate severe environmental stresses more efficiently as compared to Trichoderma viride. Our results suggest that Streptomyces IF5 can be used in place of Trichoderma viride as an effective bio-control agent. The mass production of these metabolites from Streptomyces IF5 and the mode of application on the field however have to be investigated further and the conditions are to be optimized, which would be the future direction of the present study.

Keywords : Antifungal activity, Bio-control agent, Environmental stresses, Streptomyces IF5, Trichoderma viride.