Journal of Pure and Applied MicrobiologyVol. 6 No. 4

Evaluation of Antimicrobial Activity and Characterization of Soil Actinomycetes from Social Forest Area of Bhilai Township, Chhattisgarh

Pragya Kulkarni* and Rozina Siddique

Department of Microbiology, Government V.Y.T. P.G. Auto. College, Durg - 571 602, India.

Received on 20 April 2012 and accepted on 02 June 2012

 

ABSTRACT

A total of 35 different actinomycetes strains were isolated from soil samples of social forest area of Bhilai Township. They were then evaluated for antimicrobial activity against three fungal (Aspergillus niger, Fusarium sp. and Penicillium sp.) and three bacterial strains (E.coli, Bacillus subtilis, and B.cereus). 23 isolates were found to be active against at least one of the microorganism tested by showing in vitro anti microbial activity. 09 isolates were selected as highly active antagonists for further characterization on the basis of their Total Inhibition properties. Tentative identification was done on the basis of growth on different agar medium, colour of aerial mycelium, morphology of spore mass, presence or absence of diffusible pigment and melanin, growth on different temperatures, utilization of different carbon and nitrogen sources and production of extracellular enzymes. Results were also compared with probabilistic identification method.

Keywords : Actinomycetes, Social Forest, Antimicrobial activity, Characterization.