Journal of Pure and Applied MicrobiologyVol. 6 No. 4

Comparative Profile of the Antimicrobial Activities of Assam, Dooars and Darjeeling Tea Leaves (Camellia sinensis L).

Debajyoti Mukherjee1,2, Paromita G. Bhattacharjee2 and Sutanu Samanta1*

1Lovely Professional University, NH-1, Phagwara - 144 411, India. 2Institute of Genetic Engineering, Badu, Kolkata - 700 128, India.

Received on 07 May 2012 and accepted on 07 June 2012

 

ABSTRACT

Tea, both green tea and fermented tea, have been proved to have high antimicrobial activity against many pathogenic organisms. But status of antimicrobial activity of three tea e.g. Darjeeling tea, Dooars tea and Assam tea is yet to be known. In this study, we have shown the antimicrobial activity of three types of tea against Vibrio cholerie, Pseudomonas aeruginosa, Staphylococcus aureus and Salmonella spp.All types of tea have been found to have high antimicrobial activity as all three types of tea have shown at least 1.4 cm of zone of inhibition against all the pathogenic microorganisms used in this study when 100 µl of ethanolic extract of each tea was used. Among these three types of tea, Dooars tea has highest antimicrobial activity against Pseudomonas aeroginosa and Assam tea has highest antimicrobial activity against all other microorganisms. When minimum inhibitory concentration of each tea against each microorganism have been tested, it has been found that Assam tea has the least inhibitory concentration against all the pathogenic microorganisms and it was 20µl of ethanolic concentration. So, Assam tea can be exploited as formulating drugs against those pathogens apart from its use as beverage.

Keywords : MIC, Zone of inhibition, antimicrobial activity, extract, ethanol.