Journal of Pure and Applied MicrobiologyVol. 6 No. 4

Antimicrobial Activity of Stem Bark of Phyllanthus acidus (L) Skeels and Root Bark of Croton caudatus Geiseler

Satya B. Paul and S.Saraju Devi*

Department of Chemistry, Assam University, Silchar - 788 010, India.

Received on 08 April 2012 and accepted on 07 May 2012

 

ABSTRACT

The present study was conducted to evaluate the antibacterial and antifunal activities of methanol extract of root bark of Croton caudatus Geiseler and stem bark of Phyllantus acidus (L.) Skeels at different concentration by using agar disc diffusion method. The antibacterial activities were tested against some bacterial strains (Staphylococcus aureus, Klebsiella pneumonia, Escherichia coli, Pseudomonas aeruginosa and Bascillus subtilis) and the antifungal activities were tested against some fungal strains such as Candida albicans, Tricophyton mentagrophytes, Trichosporan beigelli and Microsporum gypsum respectively. The maximum activities were observed against all the tested organisms except Bascillus subtilis, Pseudomonas aeruginosa (bacterial strains) and Microsporum gypsum (fungal strains). The minimum inhibitory concentration (MIC) also determined by using tube dilution technoque. The MIC values for methanol extract of stem bark of Phyllantus acidus (L.) Skeels ranged from 3.78-500mg/ml and the lowest MIC value (3.78mg/ml) was recorded against Staphylococcus aureus.The MIC value of root bark of Croton caudatus Geiseler ranged from 31.25-500mg/ml and the lowest MIC value (31.25mg/ml) was recorded against Klebsiella pneumonia.

Keywords : Antimicrobial activities, Phyllantus acidus (L.) Skeels, Croton caudatus Geiseler, Methanol extract, Minimum inhibitory concentration (MIC).