Journal of Pure and Applied MicrobiologyVol. 6 No. 4

Bacteriology of Diabetic Foot Ulcers

Mamatha P Samaga1 and K.M. Mythri2

1Department of Microbiology, Mandya Institute of Medical Sciences, Mandya - 571 401, India. 2Department of Microbiology, Institute of Nephrourology, Victoria Hospital Campus, Bangalore, Karnataka, India,

Received on 18 February 2012 and accepted on 21 April 2012



A prospective study was carried out on patients with diabetic foot ulcers to determine their bacteriological spectrum and to assess their in vitro susceptibility to the commonly used antibiotics. The criterion for inclusion was a diabetic patient with foot ulcer of grade 1 or more according to Meggit Wagner Classification System. Polymicrobial growth was seen where 236 bacteria were isolated with an average of 2.36 per case. Of these isolates, 218 (92.37%) were aerobes and 18(7.63%) were anaerobes. Staphylococcus aureus(22.01%) was the predominant aerobe isolated followed by Proteus mirabilis(19.7%). 16.7% of Staphylococcus aureus were methicillin resistant. All gram positive aerobes showed 100% sensitivity to vancomycin. All gram negative aerobes except Klebsiella spp. and Pseudomonas aeruginosa showed 100% sensitivity to amikacin. The major anaerobes isolated were Bacteroides spp(61.1%) followed by Peptostreptococcus spp(27.8%) and Clostridium spp.(11.1%).

Keywords : Diabetic foot ulcer, Polymicrobial, Wagner’s grades.