Journal of Pure and Applied MicrobiologyVol. 2 No. 1

Genetic analysis of Aeromonas hydrophila MTCC 646 by random amplified polymorphic DNA

S.K. Samal, B.R. Samantaray and B.K. Das*

Central Institute of Freshwater Aquaculture, P.O. Kausalyaganga, Bhubaneswar - 751 002, India,

Received on 21 November 2007 and accepted on 27 December 2007

 

ABSTRACT

RAPD profiling of Aeromonas hydrophila MTCC 646 strain was performed in order to analyze the possible genetic variability in this bacterium, the causative agent of epizootic ulcerative syndrome and hemorrhagic septicaemia. Out of one hundred sixty random decamer primers of OPA, OPB, OPC, OPE, OPF, OPJ, OPK and OPY screened against genomic DNA of A. hydrophila, sixty four amplified good, consistent and reproducible fingerprints. The greatest number of fragments was found with the use of primers OPE 15 (8 bands) and OPK 07 (9 bands). The molecular weight of the amplified products of A. hydrophila MTCC 646 against all the tested primers ranged from 0.22 kb to 3.4 kb. The clear, distinct and reproducible DNA fragments of selected primers can be used as standard for identifying new strains of A. hydrophila.

Keywords : Aeromonas hydrophila, RAPD-PCR, Genomic DNA, Primer.