Journal of Pure and Applied MicrobiologyVol. 2 No. 1

Biotransformation of Rifamycin B to Rifamycin S with free and immobilized cells of Curvularia lunata

R.H. Patil*, S.V. Patil1, J.A. Rajput2, J. Bhat3, R.G. Chaudhary4 , U.K. Patil & S.B. Chincholkar5

*Department of Microbiology, R.C.Patel Arts, Commerce and Science College, Shirpur, India. 1-4Department of Microbiology, School of Life sciences, North Maharashatra University, Jalgaon - 425 001, India.

Received on 08 February 2008 and accepted on 20 March 2008

 

ABSTRACT

Studies on Biotransformation of rifamycin B to rifamycin S revealed that immobilized mycelia of Curvularia lunata are more efficient than free cells of organism with respect to time, cultural conditions and cost. Effect of various polymers revealed that carboxymethylcellulose has stimulatory effect on biotransformation whereas other polymers do not show significant effect on biotransformation. TLC analysis of transformed product shows two bands, one matching with standard rifamycin B while another with standard rifamycin S indicates significant transformation. Optimum transformation takes place at 400c and pH7 respectively. UV-Visible spectrophotometric studies indicated that rifamycin B and rifamycin S has absorption maxima 425 and 525 respectively.

Keywords : Biotransformation, rifamycin B, rifamycin S, TLC, Curvularia lunata.