Journal of Pure and Applied MicrobiologyVol. 6 No. 1

Production of Poly-(3-hydroxybutyrate) by Bacillus sp. 112A, Utilizing some Waste Starchy Materials

M. Thirumala¹* and S. Vishnuvardhan Reddy²

¹Department of Biochemistry, Mahatma Gandhi University, Nalgonda - 508 001, India. ²Department of Plant Sciences, University of Hyderabad, Hyderabad - 500 046, India.

Received on 12 April 2011 and accepted on 30 May 2011

 

ABSTRACT

Production of poly-(3-hydroxybutyrate) by Bacillus sp. 112A isolated from activated sludge sample using flours of ten different pulses and cereals, such as, pigeon pea, red lentil, black gram, bengal gram, green gram, corn, soya bean, wheat, rice and sorghum available in abundant as spills was in a range of 1.20 g/L to 0.14 g/L. Bacillus sp. 112A was able to accumulate a maximum of 1.20 g/L PHB within 30 h of incubation when corn flour (25 g/L) was used as carbon substrate suggesting a very faster rate of polymer synthesis when compared to all other substrates.

Keywords : Bacillus sp., GC, Polyhydroxybutyrate, Production, Waste starchy materials.