Journal of Pure and Applied MicrobiologyVol. 6 No. 1

Production of Amylase using Potato Waste Under Solid State Fermentation

N. Uma Maheswari* and M. Sherley

PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College, Mannargudi - 6141 001, India.

Received on 20 April 2011 and accepted on 02 June 2011

 

ABSTRACT

Enzymes production by fermentation carried out by solid state fermentation. SSF have been established as a superior technique for the production of enzymes. The project was aimed at the production of amylase by Aspergillus niger and Bacillus subtilis using potato as substrate with the use of SSF techniques and tested for its activity. Effect of physical parameters such as concentration, temperature and pH were also assessed. Aspergillus niger was the maximum amylase producer (20.5 ± 0.02 IU/ml) compared to Bacillus subtilis using 15g of substrate concentration with an optimum pH at 4 and temperature 24oC . Substrate tested found most suitable for enzyme production and their potential could be effectively exploited in the future for production of various commercial products.

Keywords : Bacillus subtilis, Aspergillus niger, Amylase, Potato waste, Solid State Fermentation.