Journal of Pure and Applied MicrobiologyVol. 6 No. 1

Fighting the Survivor Endodontopathogen..... Enterococcus faecalis

Kavita Dube¹, Jagadish² and Vinitha³

¹Department of Endodontics, Hitkarini Dental College & Hospital, Jabalpur, India. ²Department of Endodontics, ³Department of Microbiology, R.V. Dental College & Gospital, Bangalore, India.

Received on 20 May 2011 and accepted on 05 July 2011



Root canal (endodontic) treatment is a common dental procedure performed to salvage infected teeth. However, endodontic failures are not uncommon. This study evaluated the efficacy of three intracanal medicaments against Enterococcus faecalis, the most commonly isolated organism from endodontic failure cases. The root canals of human teeth were intentionally infected with isolates of E. faecalis, following which the root canals were medicated with: Group A: Calcium hydroxide mixed with saline, Group B: Calcium hydroxide mixed with 2% Chlorhexidine and Group C: Calcium hydroxide mixed with Iodoform (Vitapex), the specimens were incubated at 370 C for 7 days. After this, the medicaments were removed from the canal by irrigation with sterile distilled water; dentinal shavings were collected from the canals which were weighed and cultured on Trypticase soy agar. The number of colony forming units per milligram (mg) was calculated and the data subjected to statistical analysis. The Calcium hydroxide – Chlorhexidine group reduced the bacterial count significantly better followed by the Calcium hydroxide – Iodoform group which showed significantly superior results in comparison to Calcium hydroxide – saline group.(p<0.001).It is concluded that the addition of Chlorhexidine to Calcium hydroxide could present an effective strategy for the treatment of teeth with post- endodontic failures.

Keywords : E. faecalis, Calcium hydroxide, Chlorhexidine, Iodoform, Endodontic, Retreatment.