Journal of Pure and Applied MicrobiologyVol. 6 No. 1

Identification of Streptomyces rochei (MMKK5_1492R_S0068) by using 16S rDNA Sequencing Isolated from Coal Fields of Andhra Pradesh

B.V. Gopinath and M.A. Singaracharya

Department of Microbiology, Kakatiya University Warangal, India.

Received on 20 June 2011 and accepted on 05 September 2011

 

ABSTRACT

Actinomycetes are best known for their ability to produce antibiotics and are gram positive bacteria which comprise a group of branching unicellular microorganisms.In this study, we revealed rapid detection of streptomycin – producing Streptomyces sp. By extraction of total soil DNA from 16 soil samples using a modified lysis method followed by PCR amplification of a genus specific sequence in the Streptomyces 16S r DNA gene. DNA band of the expected size was seen with the sample (MMKK5_1492R_S0068 (850 bp). Additionally16S rDNA fragment was amplified by PCR from MMKK-5 genomic DNA using 16S rDNA universal primers:8F and 1492R. These results indicate that PCR amplification of Streptomyces specific genes could be used for direct detection of streptomycin – producing Streptomyces rochei from coal mine soil of Andhra Pradesh

Keywords : Actinomycetes, 16 s rDNA, Amplification.