Journal of Pure and Applied MicrobiologyVol. 6 No. 1

Molecular Profiling of Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Clinical Samples

R. Mohana Priya¹, G. Rajalakshmi² and P. Pavithra³

¹Department of Biotechnology, Maharaja Co-education Arts & Science College, Perundurai, India. ²PG and Research Department of Biotechnology, Hindusthan College of Arts Science, Coimbatore. ³Kongunadu Arts amd Science College, Coimbatore, India.

Received on 12 May 2011 and accepted on 17 June 2011

 

ABSTRACT

The present study on molecular profiling was conducted at molecular level to differentiate the bacterial pathogens. The bacterium Staphylococcus aureus was isolated from five different clinical samples and were grouped as SA1, SA2, SA3, SA4 and SA5. They are confirmed as methicillin resistant Staphylococcus aureus based on their biochemical characterization. The molecular weight of plasmid DNA of the entire isolated sample was 19kb whereas the molecular weight 2400 Kb was observed in chromosomal DNA for the isolates. The genetic diversity of the isolates was also checked by restriction digestion analysis with Bam HI, Eco RI and Hind III. The enzyme Hind III with their recognition sequence 5'- AGCTT-3'digests the chromosomal and Plasmid DNA. In addition, the isolated strains were tested for their antibiotic suceptibilty patterns against amphicillin, chloramphenicol, streptomycin, rifampicin, erythromycin and methicillin. The strains were highly resistant to methicillin and susceptible to other tested antibiotics.

Keywords : MRSA- Methicillin Resistant Staphylococcus aureus, Plasmid DNA, Restriction digestion, Genetic diversity.