Journal of Pure and Applied MicrobiologyVol. 6 No. 1

Analysis and Characterization of Bioemulsifer Produced by Surfactant Degraders

Joyline Mascarenhas, Sushma Jahagirdar and Lolly Jain

Department of Microbiology, K. J. Somaiya College of Science and Commerce, Vidyavihar, Mumbai, India.

Received on 20 June 2011 and accepted on 02 September 2011

 

ABSTRACT

Out of the 5 surfactant degraders obtained from lake shore sample were grown in Mineral salt medium with different oils as substrates. The emulsification index was calculated in which Enterobacter sp. 3EAG2 showed maximum emulsification index and was further studied and partially purified by Acetone precipitation. Maximum bioemulsifier production was obtained during the stationary phase after 72 hours using almond oil as a substrate. The bioemulsifier was found to be stable at 10? C & 37 ? C. The bioemulsifier showed the presence of proteins, carbohydrates and also lipids in its backbone. The HPTLC & GC-MS analysis showed the presence of 3- hydroxyl- 2- alkyl fatty acids & saturated fatty acids, n-alkanes and esters of carboxylic acid that formed the sugar moiety respectively. The bioemulsifier showed efficient oil removing capacity and on immobilization of the Enterobacter sp. 3EAG2 cells in Sodium alginate beads, excellent detergent degradation was also observed over a period of 5 days.

Keywords : Bioemulsifier, chemical emulsifier, Immobilization, HPTLC & GCMS.