Journal of Pure and Applied MicrobiologyVol. 6 No. 1

Isolation and Characterization of Lead Resistant Micro-organism from Industrial Effluent: (A Bioremediation Approach)

Sabyasachi Chatterjee1*, Juthika Das2, Pranab Roy3 and Agniswar Sarkar4

Department of Biotechnology (Recognized by DBT- Govt. of India), The University of Burdwan, West Bengal- 713 104, India.

Received on 10 September 2011 and accepted on 02 November 2011

 

ABSTRACT

The pollution of the environment with toxic heavy metals is spreading throughout the world along with industrial progress. Microorganisms can be highly efficient bioaccumulators of soluble and particulate forms of metals. Microbe related technologies may provide an alternative or addition to conventional method of metal removal. The present study deals with isolation of lead resistant bacteria and their characterization from effluent water of Indian Iron and Steel Company (IISCO), Burnpur, West Bengal. Isolates were selected based on predominant growth on high level of heavy metal concentration. The basic characterization was done on the basis of morphological, physical, biochemical and antibiogram tests. Quantification of metal absorption was determined by Atomic absorption spectrophotometric analysis. Protein expressions of the isolates on metal stress were studied by SDS-PAGE. The identified heavy metal resistant bacteria could be useful for the bioremediation of heavy metal contaminated sewage and waste water.

Keywords : Lead resistant Bacteria, Industrial effluent, SDS-PAGE, Bioremediation.