Journal of Pure and Applied MicrobiologyVol. 6 No. 1

Study of Microbial Degradation of Caffeine from Different Samples of Coffee

Nilima S. Shivale* and Avinash Kumar

Department of Biotechnology and Bioinformatics, Padmashree Dr. D.Y. Patil University, Plot No-50 Sector – 15, C.B.D Belapur Navi Mumbai- 400 614, India.

Received on 10 June 2011 and accepted on 02 September 2011



Coffee is the most commonly consumed product worldwide. The caffeine present in coffee acts as a central nervous system stimulant. Apart from coffee, caffeine is also found in tea, yerba mate and guarana berries. It is a legal psychoactive substance available all over the world. Due to the harmful effects of caffeine on the body, the present studying aims at studying the degradation of caffeine by microorganisms so that the ill effects of the compound can be overcome. Caffaine was extracted from different types of coffee and its degradation by Bacillus subtillus and Pseudomonas aeruginosa was studied. The concentration to be used was detected by MIC and degradative activity was obtained by HPLC.

Keywords : Caffeine, Bacillus subtillus , Pseudomonas aeruginosa, MIC, HPLC.