Journal of Pure and Applied MicrobiologyVol. 6 No. 1

Production of Phenolics Under Abiotic Stress by Pisolithus tinctorius PT1 Obtained from Iron Ore Mine

Lakshangy J. Chari, Saroj N. Bhosle and Sandeep Garg*

Department of Microbiology, Goa University, Taliegao Plateau, Goa - 403 206, India.

Received on 20 April 2011 and accepted on 06 June 2011

 

ABSTRACT

Pisolithus sp. was isolated from ectomycorrhizal association with Acacia mangium growing in mining affected forest area along the west coast of India. The in vitro growth response and phenolics production by the isolate was checked using mineral medium for various edaphic factors prevailing in rejects of iron ore mines. Phosphate-citrate buffer (0.047 M) was found suitable for maintaining the pH of the medium. The isolate survived at 10 and 42oC. Interestingly, the isolate showed growth in the presence of 10,000 ppm Mn and appeared to be the most Mn-tolerant. The isolate preferred ferric form of iron over ferrous. The optima of all the variables for phenolics production were found at higher values than that required for the growth of isolate, significantly revealing the enhanced elaboration of phenolics at stress conditions. Maximum yields of phenolics obtained were 149.91, 164.07 and 61.34 µg/20ml with 1.5% NaCl, 37oC and 5% PEG, respectively. The wide tolerance and elaboration of phenolics by Pisolithus sp. to various edaphic factors present in mine rejects could be responsible for its stable existence on such disturbed site. This suggests its possible ecological application and industrial utilization.

Keywords : Metal tolerance; Mine rejects; phenolics; Phosphate inhibition; Pisolithus tinctorius.