Journal of Pure and Applied MicrobiologyVol. 6 No. 1

Functional Redundancy Diversity of Gram Positive Bacteria as Response to Pesticide (Malathion) Exposure in Soil

Hathiyah Mohammed Abu-Thiyab, Omar H.M. Shair*, Rashead M. Al-ssum, Najwa M. Aref and Bashir A. Al-ssum

Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box: 1811, 11333, Riyadh, Saudi Arabia.

Received on 04 January 2012 and accepted on 29 February 2012

 

ABSTRACT

Pesticide (Malathion) has been used extensively in Saudi Arabia when there was outbreak of the Rift Valley fever in the south region in September 2000 for elimination of the host (mosquito) and it is still in use. We studied the effect of this pesticide on treated soil. This study started in 2009 in Riyadh Saudi Arabia. Samples were collected from different locations of Riyadh area. The amounts of CO2 from soil microbial community were determined using compuflow 8650 and shown difference between the treated and untreated soil. The mean proportion of carbon dioxide of the treated was statistically and significantly lower than the untreated (3240.3 vs. 5492.6 ppm) respectively. Regression analysis revealed significant reduction of carbon dioxide equal to (b = -48.92 ppm /d) for the treated soil while for the untreated soil, the slope of regression line was stable and not significant (b = 0.37 ppm /d). BIOLOG GP2 micro plates were used to determine 95 different carbon sources substrate utilization patterns of microbial communities.

Keywords : Pesticide, Rift Valley fever, Mosquito, Soil, Microbial community.