Journal of Pure and Applied MicrobiologyVol. 6 No. 1

Influence of Circadian Variation on the Pharmacokinetics of the Components of da-cheng-qi Decoction

Juan Li, Han-Lin Gong, Yan-Yi Ren, Mei-Hua Wan, Xi Huang and Wen-Fu Tang*

Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan Province - 610 041, PR China.

Received on 29 May 2011 and accepted on 09 July 2011

 

ABSTRACT

In the present study, we investigated the effect of dosing time on the pharmacokinetics of components of the Chinese herbal formulation termed da-cheng-qi decoction (DCQD). DCQD was administered orally to rats at a dosage of 20 g/kg under a 12 h:12 h light-dark cycle at 20:00, 00:00, 08:00 and 12:00 hours (0, 4, 12 and 16 hours after light onset). Plasma samples were analyzed for aloe emodin and emodin contents by high performance liquid chromatography (HPLC). The kinetic profiles were best described by a two-compartment model. Linear regression, two-way analysis of variance(ANOVA), non-linear regression analysis, and multiple comparisons of pharmacokinetic data revealed significant dosing time variations. Times to peak concentration (Cmax) varied from 0.35 to 0.54 hours for aloe emodin and from 0.45 to 0.51 hours for emodin after administration at the selected times. Substantial time-dependent differences in the Cmax from 4.17 ± 0.82 to 0.23 ± 0.05 mg/L for aloe emodin and from 0.97 ± 0.24 to 0.27 ± 0.05 mg/L for emodin were observed. Furthermore, the area under the time-concentration curve decreased in a time-dependent manner from 7.79 ± 1.91 to 0.52 ± 0.05 mg l-1 h-1 for aloe emodin and from 2.81 ± 0.62 to 0.86 ± 0.18 mg l-1 h-1 for emodin. Findings strongly support a circadian influence on the pharmacokinetics of aloe emodin and emodin after oral dosing with DCQT, with exposure to the herbal components being greater during the rest cycle.

Keywords : Da-cheng-qi Decoction, Chronopharmacokinetics, Chinese herbal formula, HPLC.