Journal of Pure and Applied MicrobiologyVol. 6 No. 1

Isolation and Identification of Pathogenic Fungi Associated with Aloe zebrina Flower Malformation- First Report

L.R. Kido, J.D. Uzabakiriho and P.M. Chimwamurombe*

Department of Biological Sciences, Faculty of Science, University of Namibia, Private Bag 13301, 340 Mandume Ndemufayo Avenue, Windhoek, Namibia.

Received on 04 July 2011 and accepted on 11 September 2011

 

ABSTRACT

Aloe zebrina, a succulent plant, is a source of energy, food and has industrial uses. This is because of its nutritional and medicinal value in treating skin cancers and diseases. The objective of this study was to isolate and identify the fungal species associated with the malformation of the Aloe zebrina as a primary obligatory step to control the causal agents for this disease. Samples of infected Aloe zebrina flowers were collected from the gardens in the campus of the University of Namibia and pure cultures of fungi were isolated from the infected flowers using Potato Dextrose Agar media. DNA was extracted using the CTAB method and was amplified using combination of primers namely, ITS1 and ITS2, ITS1 and ITS4 and ITS3 and ITS4. The PCR products were sent for automated sequencing at Inqaba Biotec Industries in South Africa. The sequences were compared to known sequences of organisms in the Genbank by using the Basic Local Alignment Search Tool (BLAST). The BLAST searches revealed the identity of different fungi to be Nigrospora oryzae, and Arthrinium phaeospermum. This is the first report on fungal species associated with malformation of Aloe zebrina flowers and further work is on testing Koch’s postulates on these fungi.

Keywords : Aloe zebrina, Flower malformations Nigrospora oryzae, Arthrinium phaeospermum, Internal transcribed spacer.`