Journal of Pure and Applied MicrobiologyVol. 6 No. 1

Intracellular and Extracellular Fruit-Peel-Degrading Enzyme Synthesized by Rhizobium species CWP G34B

G.O. Ajayi and B. Boboye*

Department of Microbiology, P.M.B. 704, Federal University of Technology, Akure, Ondo State, Nigeria.

Received on 10 September 2011 and accepted on 28 November 2011

 

ABSTRACT

This study investigated whether the fruit-peel-degrading (FPD) enzyme synthesized by the tropical Rhizobium species CWP G34B in the presence of some fruit peels was formed intracellularly or extracellularly. The Rhizobium spp. CWP G34B was grown in a minimal medium supplemented with 1% (w/v) pectin and peels of apparently healthy mature fruits: pineapple, orange, plantain, banana, pawpaw and mango separately. Dinitrosalicylic acid and Biuret reagent methods were used to quantify glucose and protein in the grown culture and the FPD enzyme activity was based on the amounts of the substances produced by the bacterium. Intracellular enzyme activity ranged between 0.01 mg.ml-1 glucose per mg.ml-1 protein (plantain peel) and 0.05 mg.ml-1 glucose per mg.ml-1 protein (pawpaw and banana peels). The extracellular enzyme activity ranged between 0.75 mg.ml-1 glucose per mg.ml-1 protein (banana peel) and 0.86 mg.ml-1 glucose per mg.ml-1 protein (pineapple peel). The total FPD enzyme activity (0.85 mg.ml-1 glucose per mg.ml-1 protein) was highest in pawpaw peel. The study showed that there was no significant difference between the extracellular and total enzyme activities of the FPD enzyme indicating that the FPD enzyme was majorly produced extracellularly.

Keywords : Fruit-peel-degrading enzyme activity, Intracellular and Extracellular synthesis.