Journal of Pure and Applied MicrobiologyVol. 6 No. 1

Prevalence of Virulence Factor Genes in Escherichia coli Isolates from Dairy Cows with Mastitis

DaRong Cheng¹*, ShanYuan Zhu², ZhiRui Su¹, WeiYong Zuo², Jian Wang² and WeiMing Hong²

¹College of Veterinary Medicine, Yangzhou University, Yangzhou - 225 009, China. ²Jiangsu Animal Husbandry and Veterinary College, Taizhou - 225 300, China.

Received on 08 November 2011 and accepted on 24 December 2011

 

ABSTRACT

To investigate into the prevalence of virulence factor genes in mastitis-causing bacteria and prevent bovine mastitis optimistically, one hundred and twenty three Escherichia coli isolates from bovine mastitis were examined to detect the virulence associated genes by using polymerase chain reaction (PCR) methods. The data showed that 11 virulence genes were detected, including ETT2, STb, CNF2, Tra, LTa, HPI, Hly, Aer, Pfa, Sfa and F17, and none of the isolates contained the genes for Stx2e, STa, CNF1, LEE, Afa and CS31A were found. All of the detected virulence genes were present alone or in combination with each other, there into, 87 (70.73%) isolates had at least one virulence gene, and ETT2 locus was the most common gene in the examined isolates (38.21%), followed by STb (25.20%), CNF2 (19.51%), Tra (17.07%), LTa (14.63%), HPI (14.63%), Hly (7.32%), Aer (4.07%), Pfa (2.44%), Sfa (2.44%) and F17 (1.63%).

Keywords : Dairy cow, Mastitis; Escherichia coli, Virulence factor.