Journal of Pure and Applied MicrobiologyVol. 4 No. 2

Silica Cotton Ball (SCB) Technique: A cost Effective Procedure for Microbiology, Plant Pathology and Applied Pharmacology

Sandeep Acharya

Plant Physiology and Biochemistry Research Laboratory, Department of Botany, Tripura University, Suryamaninagar - 799 130, India.

Received on 05 January 2010 and accepted on 19 February 2010



For the authenticity of a new Rapid Antimicrobial Susceptibility Test (RAST) technique, antibacterial activity of Phyllanthus fraternus Webster., available in Tripura, was evaluated by a modified technique and Disc-Diffusion (DD) method. Leaf extract was prepared in ethanol solvent to be evaluated against – Xanthomonas oryzae pv. oryzae, Xanthomonas campestris pv. campestris and Ralstonia soloanacearum. Compared to Streptomycin, the leaf extract exhibited in vitro high antibacterial activity by the Silica Cotton ball technique which proves more economical than the conventional disc diffusion technique.

Keywords : Phytopathogenic bacteria, Silica-Cotton Ball disc (SCB), Disc-Diffusion (DD) method.