Journal of Pure and Applied MicrobiologyVol. 4 No. 2

Characterization and Properties of Nitrogenase Fe-protein from Rhizobium species

P. Jayalakshmi*, P. Suvarnalatha Devi, G. Revathi, N.D. Prasanna and S.K. Shaheen

Department of Applied Microbiology, SPMVV, Tirupati - 517 502, India.

Received on 03 April 2010 and accepted on 24 May 2010

 

ABSTRACT

The Nitrogenase Fe- protein was isolated from Rhizobium sps. which was purified and the properties similar to those of the Fe- Protein of other nitrogen fixing organisms with respect to its molecular weight, non-protein groups like Phosphates, Ribose and an adenine like unit are covalently bound to the protein. The nitrogense Fe protein is a a2 dimer containing Fe4S4 cluster. The Fe- protein was eluted with 400 mM- Nacl on to a DEAE-Cellulose column as a dark brown band at the top of the column. The molecular weight of the protein is about 61500Da.

Keywords : Nitrogenase Fe- protein, Rhizobium sps, DEAE-cellulose column, NifH gene.