Journal of Pure and Applied MicrobiologyVol. 4 No. 2

Study on the Amylolytic Activity of Bacteria Isolated from Flour Mill Waste

CH. Mina Kumari, I.Bhaskar Reddy and K. Vijaya Rachel*

Department of Biochemistry, GIS, GITAM University, India.

Received on 30 March 2010 and accepted on 08 May 2010



The present investigation deals with the screening and study on the amylolytic activity of bacteria isolated from flour mill waste. Six different bacterial colonies were isolated and tested for starch hydrolysis. Out of them, isolate no A4 has showed maximum zone of hydrolysis of 3.3 mm in starch agar medium and identified as Bacillus. The isolated Bacillus was subjected for amylase assay and extra cellular amylase production. The maximum amylase production was achieved with a reducing sugar concentration of 2.25 IU/ml at 37 0C for 48 hours of incubation.

Keywords : Bacillus, amylase, starch hydrolysis, extra cellular amylase.