Journal of Pure and Applied MicrobiologyVol. 4 No. 2

Antioxidant and Antibacterial Potentials of Aloe vera Juice Extract against Wound Isolates

M. Ranjani¹, S. Rajan¹ and P. Brindha²

¹Departmant of Microbiology, Srimad Andavan Arts and Science College, Thiruchirappali - 620 005, India. ²CARISM, SASTRA University, Thanjavur, Tamilnadu, India.

Received on 27 March 2010 and accepted on 02 May 2010

 

ABSTRACT

Wound is a injury made on the skin due to torn, cut, trauma and contusion. Microorganisms are responsible for the severity in hospitalized wound. To evaluate the importance of Aloe vera on the wound pathogens this work was undertaken. The healing properties of the succulent plant Aloe vera have been known for thousands of years. This study was aimed at to evaluate the antibacterial and antioxidant activity of Aloe vera extract. Wound (pus) samples were collected from Government hospital Srirangam. Samples were processed and the microorganisms were isolated and identified using macroscopic, microscopic and biochemical analysis. Escherichia coli, Staphylococcus aureus, Streptococcus pyogens and Pseudomonas aeruginosa were isolated and identified as a predominant flora of wound infection. Aloe vera plant was chosen for screening its antibacterial activity. Antibacterial activity of Aloe vera extract was studied by disc diffusion method, which showed best activity against all clinical isolates at 200µg/ml concentration. Phytochemical analysis of the plant extract revealed the presence of flavonoids, tannins, phenolic compounds and steroids.

Keywords : Aloe vera, wound, Pus, Antimicrobial activity, Antioxidants, Phytochemistry.