Journal of Pure and Applied MicrobiologyVol. 4 No. 2

Isolation and Molecular Characterization of Aeromonas from Food Animals by PCR method

Indu Sharma¹*, A.K. Pramanik² and A. Kumar³

¹Department of Microbiology, Assam University, Silchar - 788011, India ²Department of Veterinary Public Health, West Bengal University of Animal and Fishery Sciences, Kolkata - 700 037, India. ³Department of Veterinary Public Health, IVRI, Izat nagar, India.

Received on 18 January 2010 and accepted on 24 February 2010

 

ABSTRACT

A total of 332 samples of animal food origin comprising 104 poultry, 137 fish, 51 pork and 40 goat (chevon) were screened. Molecular typing of Aeromonas isolates revealed that all the 38 isolates belonged to Aeromonas spp. and were positive for 16S rRNA (100%), ahh1 (60.52%), asa1 (42.10%), A. hydrophila aerA (13.15%) and AHCYTOEA/aerA (5.26%). It was noted that aerolysin producing toxin genes were the most prevalent irrespective of their geographical locations and the most common single gene carried among all the isolates examined was ahh 1 (60.52%).

Keywords : Aeromonas, PCR, Food animals.