Journal of Pure and Applied MicrobiologyVol. 4 No. 2

16S rDNA sequence for Identification and Discriminating Pseudomonas aeruginosa

J.R. Parvathi*, S.D. Sunita and B.K. Vasudha

Department of Biotechnology and Bioinformatics, Padmashree Dr. D.Y. Patil University, Plot No-50 Sector-15, C B D Belapur, Navi Mumbai - 400 614, India.

Received on 21 March 2010 and accepted on 07 May 2010

 

ABSTRACT

Pseudomonas aeruginosa is emerging, as one of the leading causes of nosocomial infection. It’s ubiquitous nature and pathogenicity is attributed to their enormous genome that encrypts for virulence, antibiotic resistance and other regulatory genes which are fundamental for the survival and growth of this bacterium. A comparative analysis of Random Amplified Polymorphism across genomic DNA (RAPD) and ribosomal DNA (RArDNA) with Amplified Ribosomal DNA Restriction Analysis (ADRDA) targeting 16S rDNA region, were attempted in typing Pseudomonas aeruginosa isolates obtained from clinical (UTI) and diverse environmental sources. RAPD proved to be a better typing technique than RArDNA and ADRDA, though none of these techniques gave conclusive patterns for clinical samples. RArDNA gave unique patterns for only 3 environmental samples. ADRDA gave restriction pattern with BamH1 only. An amplicon of 956 base pairs by 16S rDNA-PCR for Oxidase and Gelatin negative Pseudomonas aeruginosa strains established the superiority of molecular assay in bacterial identification

Keywords : Random Amplified Polymorphism across genomic DNA, Amplified Ribosomal DNA Restriction Analysis