Journal of Pure and Applied MicrobiologyVol. 4 No. 2

Comparative Hydrolytic Properties of Culture Filtrate of Trichoderma viride Fd18 and Aspergillus ustus Fd12 on Lignocellulosic Substrates

Mohammed Inuwa Ja’afaru* and Abiodun Anthony Onilude

Microbial Physiology and Biotechnology Research Group, Department of Botany and Microbiology, University of Ibadan, Nigeria.

Received on 12 August 2009 and accepted on 19 October 2009

 

ABSTRACT

This paper reports the enzymatic hydrolysis of alkali-treated agri-waste with xylanase from Trichoderma viride Fd18 and Aspergillus ustus Fd12 isolated from saw dust. Alkali-treated wheat bran had the highest hydrolysis rate of 35% (A. ustus Fd12 xylanase) and 48.6% (T. viride Fd18 xylanase). Saw dust from different plants have a low rate of hydrolysis. The optimal pH and temperature for hydrolysis of wheat bran by xylanase from the 2 fungi were pH 4.0 – 4.5 and 35oC. A wheat bran concentration of 6% and incubation time of 24hr increased rate of hydrolysis. The enzyme can be used to hydrolyze xylan in agricultural residues for commercial applications.

Keywords : Wheat bran, Trichoderma viride, Aspergillus ustus, hydrolysis, xylan, xylanase.