Journal of Pure and Applied MicrobiologyVol. 4 No. 2

Evaluation of the Efficacy of Maternal Vaccination by Using Dominant Pathogenic Escherichia coli Isolates to Control Neonatal Diarrhea in Individual Swine Farm

DaRong Cheng¹*, ShanYuan Zhu², WenWei Ding³, XiaoLang Chen¹, XiaoPan Gao¹ and HuaiChang Sun¹

¹College of Veterinary Medicine, Yangzhou University, Yangzhou - 225 009, China. ²Jiangsu Animal Husbandry and Veterinary College, Taizhou - 225 300, China. ³Xuzhou Bio-engineering Higher Vocational School; Xuzhou - 221 006, China.

Received on 30 April 2010 and accepted on 25 June 2010

 

ABSTRACT

As the young mammal is unable to develop efficiently its own local immune responses in the first few days after birth, the passive mucosal protection of neonate piglets is dependent on the continuous supply until weaning of maternal immunoglobulin. To deep evaluate the efficacy of maternal vaccination in swine, in this research, virulence factor genes of prevalent E. coli isolates from diarrheic piglets were determined, and two dominant pathogenic Escherichia coli isolates were screened out and inactivated to use as antigens to vaccinate pregnant sows. The maternal immunity was evaluated based on the titers of antigen-specific milk antibodies, brush border cells adherence inhibition and the passive protective efficacy of newborn piglets. It was confirmed that maternal immunization could result 2 weeks in the appearance of F4- and F6- specific antibodies in the milk. In addition, the immune colostrum exhibited the ability to inhibit the binding of F4- and/or F6-positive E. coli to porcine brush borders, while the control samples without the blocking action. Furthermore, maternal vaccination could significantly reduce the diarrhea incidence rate from 55.5% to 15.2% and evidently postpone the disease time from day 6.32 to 12.29 in average.

Keywords : Piglet; Neonatal diarrhea; Maternal vaccination.