Journal of Pure and Applied MicrobiologyVol. 3 No. 2

Production of Poly-3-hydroxybutyrate from Inexpensive Substrates

M. Thirumala*, S. Vishnuvardhan Reddy and S.K. Mahmood

Environmental Microbiology Lab, Department of Botany, Osmania University, Hyderabad- 500 007, India.

Received on 04 May 2009 and accepted on 27 June 2009



Bacillus megaterium 79/A, a starch and lactose utilizing strain, produced 0.90 g/l PHB with corn flour as sole carbon substrate; this was followed by other flours like, soya bean, wheat etc. Bacillus megaterium 79/A produced 0.62 g/l PHB with treated whey of 46.2% concentration and 0.55 g/l PHB with the same concentration of untreated whey. When 5mM microcosmic salt was used as an additional supplement, the organism produced 0.95 g/l PHB and 0.80 g/l PHB with 46.2% treated whey and 46.2% untreated whey, respectively.

Keywords : Bacillus megaterium, Lactose, PHB, Starch, Whey.