Journal of Pure and Applied MicrobiologyVol. 3 No. 2

Herbicide Induced Reversion to Prototrophy of a Non-Sporulating Mutant of Anabaena doliolum

Anupma Srivastava¹ and A.K. Pandey²*

¹Department of Botany, Shri A.K.A.P.G. College, Varanasi, India. ²Biological Research Lab, Department of Botany and Biotechnology, Kutir P.G. College, Chakkey - 222 146, Jaunpur, India.

Received on 10 April 2009 and accepted on 23 July 2009

 

ABSTRACT

Spontaneous and MNNG-induced reversion of non-sporulating mutant (Ad-Ns, het+ nif+ sp-) of the cyanobacterium A. doliolum, to prototrophy (Ad-S,het+ nif+ sport+) was investigated to compare the mutagenic potential of the herbicide alachlor. Alachlor induced reversion to protrophy was greater than spontaneous and MNNG-induced frequencies. The prototrophic revertants, either spontaneous or MNNG and alachlor induced, of non-sporulating mutants have a heterocyst frequency of 5.1±0.6% as in parent. Sporulation started during stationary phase of the growth of the revertants (Ad-S, het+ nif+ sport+) and spore frequency reached upto 92.0±0.25% against parents frequency (100%) the growth pattern of the revertant was slightly and significantly slower than the parent in N2-medium.

Keywords : Cyanobacterium, Revertants, Prototrophy, Heterocyst, Sporulation, Herbicide.