Journal of Pure and Applied MicrobiologyVol. 3 No. 2

Insecticidal Effect of Beauveria Bassiana and Paecilomyces fumosoroseus alone and in Combination with Diatomaceous Earth Against Rice Weevil (Sitophilus oryzae) and Red Flour Beetle (Tribolium castaneum)

K. Ramaswamy*, N. Vijayarangan and H.E. Krishnaiah

Food Protectants and Infestation Control Department, Central Food Technological Research Institute (CSIR), Mysore- 570 020

Received on 06 May 2009 and accepted on 09 June 2009



The virulence (bioassay tests) of two fungal entomopathogens such as Beauveria bassiana and Paecilomyces fumosoroseus alone and in combination with 250 and 500 ppm of Diatomaceous earth (DE) was evaluated at laboratory scale. The results demonstrate that 50% relative humidity (RH) is more suitable to kill the insects than at 70% RH. Further, combined inoculation treatments of both the entomopathogenic fungi under test, along with higher doses of DE (500 ppm), recorded greater mortality than any other treatments followed by combined treatment of DE at 250 ppm. Nevertheless, the control treatments have recorded from 10-18% mortality. Although higher % mortality was observed with single treatment of Beauveria bassiana and Paecilomyces fumosoroseus over controls, greater differences were not observed compared to combined treatment of Beauveria bassiana with 250 ppm of DE. However, simultaneous use of DE with the fungus resulted an additive effect. Therefore, from the practical point of view, this substance (DE) is very good alternative to traditional insecticides.

Keywords : Entomopathogens, Diatomaceous earth (DE), Bioassay, Relative humidity (RH)