Journal of Pure and Applied MicrobiologyVol. 3 No. 2

The Potential Application of Keratinase from Bacillus sp. as Plant Growth Promotors

V. Thirumalai Arasu¹*, T. Sivakumar¹, V. Ramasubramanian², K. Nalini¹ and R. Kiruthiga¹

1Department of Microbiology and 2Department of Plant Biology and plant Biotechnology, Ayya Nadar Janaki Ammal College, Sivakasi - 626 123, India.

Received on 26 June 2009 and accepted on 04 August 2009

 

ABSTRACT

Keratinolytic Bacillus sp. AJ4 and Bacillus sp. AJ9 isolated from feather dumped soil produced keratinase of 82 U/ml (specific activity of 37.3 U/mg) and 78 U/ml (specific activity of 34.2 U/mg), respectively at pH 7 and 10. Keratinase of Bacillus sp. AJ4 exhibited increased stability (82-94% and ~50%) in Triton X-100 and H2O2. Bacillus sp. AJ9 keratinase was 100% stable in SDS. The rice seeds treated with feather hydrolysate showed 30% increased vigour index. Significant stability towards detergents, improved feed conversion ratio and plant growth confirms the suitability of keratinase from Bacillus sp. AJ4 and AJ9 for agricultural applications.

Keywords : Bacillus sp., Keratinase, Vigour index, PGPR.