Journal of Pure and Applied MicrobiologyVol. 3 No. 2

Cloning of a Hypothetical NiCoT gene of Neurospora crassa

B. Sri Ramya, P. Janaki Ramayya and S.A. Mastan*

Post Graduate Department of Biotechnology, P.G. Courses & Research Centre, D.N.R. College, Bhimavaram, West Godavari Dt - 534 202, India.

Received on 18 February 2009 and accepted on 21 April 2009

 

ABSTRACT

In the present investigation attempts have been made to clone the NiCoT gene of N.crassa. For this purpose, the RNA was isolated from N. crassa and it was converted into cDNA. The nct gene was amplified using the gene specific primers. The genomic and protein sequences of nct genes have traced out using the FCGS web page and the sequences have been reported. It has also been observed that the rate of transportation in N.crassa is enhanced by the action of NiCoT gene. The transmembrane domains of the nct gene were identified using the TMpred software.

Keywords : Cloning, Nickel, Cobalt, NiCoT transporter gene, Neurospora crassa.