Journal of Pure and Applied MicrobiologyVol. 3 No. 2

Isolation of Bacterial Strains Degrading High Concentration of Phenol from Wastewater Contaminated Sites

K. Veenagayathri and N. Vasudevan

Centre for Environmental Studies, Anna University, Chennai - 600 025, India.

Received on 18 February 2009 and accepted on 21 April 2009



Bacterial strains were isolated from wastewater contaminated sites, which had the ability to utilize higher concentration of phenol of 1000 mg/L as carbon and energy source at neutral pH under aerobic conditions. Phenol degrading strains were isolated by employing enrichment culture technique a basal salt (BS) medium supplemented with different phenol concentrations as a sole carbon source. The results showed that some of the strains could use phenol as sole carbon source at very higher concentration. Several strains were isolated of which only six strains were able to tolerate very high concentration of phenol. The isolates were Pseudomonas cepacia, P. fluorescens, Pseudomonas sp., P. stutzeri and P. putida. The strains had additional abilities to degrade catechol and resorcinol. Of the six strains Pseudomonas putida was able to degrade phenol (1000mg/L) individually up to 89%.

Keywords : Biodegradation, Phenol, Wastewater.