Journal of Pure and Applied MicrobiologyVol. 3 No. 2

Isolation of Bifidobacterium from Infant Feces and its Characterization

T.R. Angeline Shimly, R. Sridar and P. Logeshwaran

T.R. Angeline Shimly, R. Sridar and P. Logeshwaran

Received on 30 March 2009 and accepted on 04 June 2009

 

ABSTRACT

Bifidobacteria are the predominant species in the feacal microflora of breast fed infants is due to the bifidigeneic factors. The efficient isolates of Bifidobacteria were selected, based on their probiotic characteristics. Isolate BAL I was able to grow under acidic condition of pH 3.5 and 4.5, resistant against bile salt at 0.5 per cent and resistant to antibiotic Cholaramphenicol (30 mcg), Ampicillin (10 mcg) and Gentamycin (10 mcg). The isolate BAL I was found to show maximum inhibitory activity against E.coli (10mm) compared to Bifidobacterium bifidum (ATCC 29521) which recorded 8mm of inhibition zone. The integration of bifidobacteria in dairy and poultry products is of interest for reinstalling the intestinal microflora.

Keywords : Bifidobacterium, Infant feces, Antibiotics.