Journal of Pure and Applied MicrobiologyVol. 3 No. 2

Media Engineering for the Production of Cellulases by a Novel Strain Aspergillus sp. IICT-F 141 using Wheat Bran as Raw Material

J. Vanajakshi, Ch. Subhakar and Annapurna Jetty*

Bioengineering and Environmental Center, Indian Institute of Chemical Technology, Hyderabad - 500 007, India.

Received on 11 April 2009 and accepted on 09 May 2009

 

ABSTRACT

Fifteen different fungal strains were isolated from sawdust samples and tested for cellulase production. Out of them one fungal strain namely Aspergillus sp. IICT–F 141 was able to produce maximum cellulase by submerged fermentation. Wheat bran was used as a substrate for the production of cellulolytic enzymes in batch culture using shake flasks. Optimizations of fermentation medium parameters such as wheat bran particle size, concentration, pH, organic and inorganic nitrogen sources were studied to enhance the further production of cellulases. From the present optimization studies organic nitrogen sources and pH have played a major role by enhancing the celluolytic activity from 34.67 to 184.28 and 180.49 IU/ml of CMCase activities respectively. The overall increase in the production was observed to be 5 fold for both CMCase and FPase, when compared to the pre optimized conditions.

Keywords : Cellulase, Aspergillus sp, Wheat bran, Particle size, Optimization.