Journal of Pure and Applied MicrobiologyVol. 2 No. 1

Microbial Growth in Stored Human Breast Milk

O.F. Olukunle* and F.C. Adetuyi

Department of Microbiology, Federal University of Technology, P.M.B 704, Akure, Ondo State, Nigeria.

Received on 11 February 2008 and accepted on 03 April 2008



Investigation into the effect of storage period and temperature on the shelf life of Human Breast Milk (HBM) was carried out to assess the quality and fitness of expressed HBM after storage for 3 and 6 hours at 4C and 26C. The control sample was collected from a nursing mother using standard hygienic method while other samples were collected aseptically from nursing mothers, day care centers, cr?ches and various homes. The samples were conveyed to the laboratory for microbial analyses. There was no fungal growth observed in any of the samples. The mean bacterial count obtained for the fresh HBM was 1.53 103 and 1.85 x 103 Cfu/mL at 4C and 26C respectively after 3 hours of storage. At 4C the bacterial load was 1.61 x 103 Cfu/mL and 2.16 x 103 at 26C after 6 hours. Before and after 3 hours of the storage at 4C, the control HBM had no microbial load, but has a value of 0.4 103 Cfu/mL at 26C after 6 hours. The bacteria isolated from milk samples after storage are Bacillus subtilis, Corynebacterium spp., Lactobacillus salivarus, Staphylococcus aureus, Staph. epidermidis, Lactobacillus acidophilus, Neisseria catarrhalis and Streptococcus lactis. It is recommended that the length of storage should not exceed 6 hours at 26C and 24 hours at 4C.

Keywords : Microbial Growth, Human Breast Milk.