Journal of Pure and Applied MicrobiologyVol. 2 No. 1

Cadmium resistant actinomycetes isolated from agricultural fields

Syed G. Dastager1-3, Wen-Jun Li2, D. Agasar3, Jae-Chan Lee1, Dong-Jin Park1 and Chang-Jin Kim1

1Korea Research Institute of Bioscience and Biotechnology (KRIBB)-Daejeon - 305 333, Korea. 2Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan - 650 091, India. 3Department of Studies and Research in Microbiology, Gulbarga University, Gulbarga - 585 106, India.

Received on 21 November 2007 and accepted on 27 December 2007

 

ABSTRACT

Two actinomycetes strains designated as DAS 131 and DAS 165, were isolated from cadmium contaminated agricultural soil of Gulbarga, Karnataka. The isolates were classified as Streptomyces spp. A plate diffusion assays showed to be resistant to cadmium [Cd (No3)2]. This method allowed a qualitative screen of the effects of metal on growth, on sporulation and in some cases, on secondary metabolic production. The strains have the best growth capacity in presence of 100mM Cdl-1 which is representative of their ability to soil colonization.

Keywords : Streptomyces; bioremediation; metal resistance.