Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Effects of Pyramid Viper, Echis pyramidum Crude Venom on Hepatic Redox Status and BAX Expression in Rats

Awadh M. Al-Johany1, Mohamed K. Al-Sadoon1*, Ahmed E. Abdel Moneim2, Amira A. Bauomy2 and Marwa S.M. Diab3

1Department of Zoology, College of Science, King Saud University, Saudi Arabia. 2Department of Zoology & Entomology, Faculty of Science, Helwan University, Cairo, Egypt. 3Molecular Drug Evaluation Department, National Organization for Drug Control & Research (NODCAR), Giza, Egypt.

Received on 15 August 2014 and accepted on 07 October 2014

 

ABSTRACT

In the present study, we have investigated the Echis pyramidum crude venom induced oxidative stress and apoptosis after 2, 4 and 6 hrs in livers of rats injected with the [LD50 and µ LD50. Wistar rats were randomly divided into 3 groups, the control group was intrapretoneal (i.p.) injected with saline while, [LD50 and µ LD50 doses envenomed groups i.p. injected with venom at a dose of 0.6325 and 1.265 mg/kg body weight, respectively. Animals were sacrificed after 2, 4 and 6 hrs from the injection. Lipid peroxidation, nitric oxide and glutathione levels as oxidative markers were measured in liver homogenate. In addition, liver functions parameter and activity of catalase were determined. E. pyramidum crude venom increased lipid peroxidation and nitric oxide production in liver with concomitant reduction in glutathione, catalase, and total antioxidant capacity. These findings were associated with apoptosis induction in the liver as indicated with the increment in BAX expression. In addition, E. pyramidum crude venom caused hepatic injury as indicated by histopathological changes in the liver tissue with an elevation in total bilirubin, serum alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase. On the basis of the present results it can hypothesized that E. pyramidum crude venom is a potent toxins-mediated hepatotoxicity associated with apoptosis induction in the hepatic tisue.

Keywords : Echis pyramidum venom; Hepatotoxicity; Oxidative stress; BAX; Rats.